About Tergitol-7 Agar Base
Key Features & Composition
- Intended Use: Selective enumeration and identification of coliform organisms.
- Key Ingredients: Proteose peptone, yeast extract, lactose, sodium heptadecyl sulphate (Tergitol-7), and bromothymol blue.
- Selective Action: Tergitol-7 inhibits gram-positive bacteria and stops Proteus swarming, helping coliforms grow better.
- Indicator: Bromothymol blue acts as a pH indicator to show lactose fermentation.
- Optional Additive: Triphenyl Tetrazolium Chloride (TTC) solution can be added to help identify specific bacteria like Escherichia colKey Features & Composition
- Intended Use: Selective enumeration and identification of coliform organisms.
- Key Ingredients: Proteose peptone, yeast extract, lactose, sodium heptadecyl sulphate (Tergitol-7), and bromothymol blue.
- Selective Action: Tergitol-7 inhibits gram-positive bacteria and stops Proteus swarming, helping coliforms grow better.
- Indicator: Bromothymol blue acts as a pH indicator to show lactose fermentation.
- Optional Additive: Triphenyl Tetrazolium Chloride (TTC) solution can be added to help identify specific bacteria like Escherichia colKey Features & Composition
- Intended Use: Selective enumeration and identification of coliform organisms.
- Key Ingredients: Proteose peptone, yeast extract, lactose, sodium heptadecyl sulphate (Tergitol-7), and bromothymol blue.
- Selective Action: Tergitol-7 inhibits gram-positive bacteria and stops Proteus swarming, helping coliforms grow better.
- Indicator: Bromothymol blue acts as a pH indicator to show lactose fermentation.
- Optional Additive: Triphenyl Tetrazolium Chloride (TTC) solution can be added to help identify specific bacteria like Escherichia colKey Features & Composition
- Intended Use: Selective enumeration and identification of coliform organisms.
- Key Ingredients: Proteose peptone, yeast extract, lactose, sodium heptadecyl sulphate (Tergitol-7), and bromothymol blue.
- Selective Action: Tergitol-7 inhibits gram-positive bacteria and stops Proteus swarming, helping coliforms grow better.
- Indicator: Bromothymol blue acts as a pH indicator to show lactose fermentation.
- Optional Additive: Triphenyl Tetrazolium Chloride (TTC) solution can be added to help identify specific bacteria like Escherichia colKey Features & Composition
- Intended Use: Selective enumeration and identification of coliform organisms.
- Key Ingredients: Proteose peptone, yeast extract, lactose, sodium heptadecyl sulphate (Tergitol-7), and bromothymol blue.
- Selective Action: Tergitol-7 inhibits gram-positive bacteria and stops Proteus swarming, helping coliforms grow better.
- Indicator: Bromothymol blue acts as a pH indicator to show lactose fermentation.
- Optional Additive: Triphenyl Tetrazolium Chloride (TTC) solution can be added to help identify specific bacteria like Escherichia colKey Features & Composition
- Intended Use: Selective enumeration and identification of coliform organisms.
- Key Ingredients: Proteose peptone, yeast extract, lactose, sodium heptadecyl sulphate (Tergitol-7), and bromothymol blue.
- Selective Action: Tergitol-7 inhibits gram-positive bacteria and stops Proteus swarming, helping coliforms grow better.
- Indicator: Bromothymol blue acts as a pH indicator to show lactose fermentation.
- Optional Additive: Triphenyl Tetrazolium Chloride (TTC) solution can be added to help identify specific bacteria like Escherichia col
Selective Identification of ColiformsTergitol-7 Agar Base is engineered to promote the growth of coliform bacteria while inhibiting unwanted organisms, making it an essential tool in water, food, and environmental microbiology laboratories. Its formulation ensures reliable differentiation based on characteristic colony morphologies and color development, facilitating targeted analysis.
Simple and Reliable Media PreparationPreparing this agar base is straightforward: dissolve the specified amount in purified or distilled water, then boil and autoclave to achieve complete sterilization. These uncomplicated steps ensure the medium is ready for inoculation and subsequent microbial cultivation, saving time while maintaining reliability and consistency.
Proper Storage and LongevityEnsure maximum effectiveness by storing Tergitol-7 Agar Base in a tightly closed container, away from moisture and direct sunlight. Adhering to the recommended storage conditions preserves the integrity and performance of the medium, supporting accurate results throughout its shelf life.
FAQ's of Tergitol-7 Agar Base:
Q: How should Tergitol-7 Agar Base be prepared for laboratory use?
A: To prepare Tergitol-7 Agar Base, suspend 33.13 grams of the powder in 1000 ml of purified or distilled water. Boil gently to dissolve completely, then sterilize the mixture by autoclaving at 121C for 15 minutes. Allow to cool and pour into sterile petri dishes before inoculation.
Q: What is the recommended incubation condition for Tergitol-7 Agar Base?
A: Once inoculated, plates should be incubated at 35C for 24 hours. This temperature and timeframe optimize the selective enumeration and identification of coliform organisms.
Q: Where is Tergitol-7 Agar Base typically used?
A: This medium is primarily used in microbiology laboratories for the selective detection and differentiation of coliform bacteria in water, food, and environmental samples.
Q: What are the benefits of using Tergitol-7 Agar Base over other media?
A: Tergitol-7 Agar Base selectively inhibits non-coliform bacteria, enhancing the isolation of target organisms and improving the accuracy of enumeration and identification processes.
Q: How should Tergitol-7 Agar Base be stored to maintain its quality?
A: Store the powder tightly closed in a cool, dry place, protected from moisture and direct sunlight. This preserves the medium's effectiveness and extends its usability.
Q: What is the physical appearance and pH level of Tergitol-7 Agar Base?
A: The medium appears as a cream to light green, homogeneous, free-flowing powder with a pH of 6.9 0.2 at 25C, making it suitable for standard microbiological applications.