About Sulphanilic Acid 0.8% Himedia
R015 (Sulphanilic Acid, 0.8%) is an analytical liquid reagent used alongside -Naphthylamine solution (R009) to detect nitrate reduction in bacteria like
Enterobacteriaceae. Nitrate reducers turn nitrate into nitrite, which reacts with this reagent to create a visible pink-red azo dye.
Key Application: Nitrate Reduction TestSulphanilic Acid 0.8% is primarily formulated to facilitate nitrate reduction tests in microbiological and biochemical laboratories. Its precise concentration and stable pH ensure reliable results during the detection of nitrate-reducing microorganisms. This process is essential for both research and clinical laboratories where accuracy is paramount.
Product Features and HandlingThis solution comes as a clear to slightly yellow liquid in a secure 100 mL bottle, maintaining its purity over time. The product is engineered for user safety and convenience with straightforward storage requirements-below 30C in a tightly closed container. The liquid formulation simplifies its use in routine analytical workflows.
FAQ's of Sulphanilic Acid 0.8% Himedia:
Q: How do I use Sulphanilic Acid 0.8% solution in a nitrate reduction test?
A: To use Sulphanilic Acid 0.8%, add a measured amount of the solution to your test sample as directed in the nitrate reduction protocol. The reagent reacts with nitrite, forming a colour change that helps interpret the results. Always follow standard laboratory instructions for precise methodology.
Q: What are the main benefits of using Sulphanilic Acid 0.8% from Himedia?
A: This reagent provides high reliability for microbiological and biochemical analysis, boasting consistent purity (0.8% w/v) and a carefully maintained pH (1.50-1.70). Its ready-to-use liquid form reduces preparation time and enhances reproducibility in nitrate reduction assays.
Q: When should Sulphanilic Acid 0.8% solution be used or replaced?
A: Use the solution before its two-year shelf life expires, and always check for any signs of discoloration or contamination. Store it below 30C in a tightly closed bottle to preserve its efficacy. Replace the solution if its appearance changes or after the indicated expiration date.
Q: Where should I store the Sulphanilic Acid 0.8% solution?
A: Store the solution in a cool, dry place below 30C. Ensure the container is tightly closed and kept away from direct sunlight or heat sources to maintain reagent stability and effectiveness throughout its shelf life.
Q: What is the physical appearance and composition of the product?
A: Sulphanilic Acid 0.8% is provided as a clear to pale yellow liquid solution, with a concentration of 0.8% w/v. It is supplied in a 100 mL sealed bottle for safe transport and storage.
Q: Why is Sulphanilic Acid 0.8% particularly suitable for microbiological analysis?
A: Its standardized purity and pH, along with stringent manufacturing standards, make Sulphanilic Acid 0.8% dependable for detecting nitrate reduction-an essential process for identifying certain bacterial species in microbiological workflows.